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Image Search Results
Journal: Journal of Immunology (Baltimore, Md. : 1950)
Article Title: Notch Ligand Delta-Like 4 Blockade Alleviates Experimental Autoimmune Encephalomyelitis by Promoting Regulatory T Cell Development
doi: 10.4049/jimmunol.1100725
Figure Lengend Snippet: Dll4-mediated signaling regulates Treg expansion in vivo and in vitro. EAE was induced in C57BL/6 mice immunized with MOG(35–55)/CFA and treated with anti-DLL4 blocking Ab or control IgG every other day and for a total of five injections (arrows) starting on the day of the immunization. A, Flow cytometry plots from individual mice from each group for CD4 and Foxp3 staining of splenocytes or lymph node cells on DPI 10 (preclinical disease) and spinal cord cells on DPI 14 (peak of disease). Results are representative of three independent experiments with five mice per group. B, Statistical analysis of staining described in A. *p < 0.05, **p < 0.005 by unpaired t test. CD4+Foxp3− T cells (C) or total CD4+ T cells (D) were isolated from naive Foxp3.GFP.KI mice and stimulated in vitro with plate-bound anti-CD3 and anti-CD28 (1 µg/ml) in the presence of plate-bound rDll4 or control IgG (2 ug/ml). Optimal dose TGF-β (3 ng/ml) or IL-2 (20 ng/ml) was added to the culture medium when indicated and then supplemented to the culture medium on day 2 of culture. Cells were washed and stained for CD4 and Foxp3 on day 4 of culture. Results are representative of three independent experiments.
Article Snippet: Recombinant mouse Dll4-Fc fusion protein, IL-2, and
Techniques: In Vivo, In Vitro, Blocking Assay, Flow Cytometry, Staining, Isolation